Markov processes in single molecule fluorescence

SnapShot: Single-Molecule Fluorescence

Common uorescent agents include organic uorophores (e.g., Cy5), uorescent proteins (e.g., GFP), and semiconductor quantum dots each with different size, photobleaching, blinking, and labeling ef ciency properties. Wideeld detection with EMCCD camera enables parallel measurements. The temporal resolution is >ms, and the working concentration is limited to nM or less. Confocal detection from an e...

Single-molecule fluorescence spectroscopy in (bio)catalysis.

The ever-improving time and space resolution and molecular detection sensitivity of fluorescence microscopy offer unique opportunities to deepen our insights into the function of chemical and biological catalysts. Because single-molecule microscopy allows for counting the turnover events one by one, one can map the distribution of the catalytic activities of different sites in solid heterogeneo...

Single-Molecule FISH ▶ Single-Molecule RNA FISH Single-Molecule Fluorescence Resonance Energy Transfer

In this article, we review smFRET, a powerful technique for measuring distances and monitoring dynamics at the molecular scale. In this technique, the researcher monitors distances between two or more individual fluorescent labels by measuring how efficiently electronic energy is transferred between them, a phenomenon known as ▶ F€orster resonance energy transfer (FRET). Its power lies in its a...

The KTH Visit in Semi-Markov Processes

Plasmonic band structure controls single-molecule fluorescence.

Plasmonics and photonic crystals are two complementary approaches to tailor single-emitter fluorescence, using strong local field enhancements near metals on one hand and spatially extended photonic band structure effects on the other hand. Here, we explore the emergence of spontaneous emission control by finite-sized hexagonal arrays of nanoapertures milled in gold film. We demonstrate that al...